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By De Cicco V., Fusco N., Verde A.

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Fluorescence polarization decreases as the excited state lifetime of the dye (τ) increases. Note that these simulations assume that the dye is rigidly attached to a spherical carrier. 4 Dyes for Fluorescence Polarization Assays Tracers used in fluorescence polarization assays include peptides, drugs and cytokines that are modified by the attachment of a fluorescent dye. Depolarization due to flexibility in the attachment of the dye, sometimes referred to as the “propeller effect,” distorts the relationships between P and molecular weight shown in Figure 2.

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A chain rule formula in BV and application to lower semicontinuity by De Cicco V., Fusco N., Verde A.


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